microbetracker matlab software Search Results


microbetracker  (MathWorks Inc)
90
MathWorks Inc microbetracker
Microbetracker, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microbetracker software  (MathWorks Inc)
90
MathWorks Inc microbetracker software
Association of TopoIV subunits ParC and ParE with MukBEF in vivo . (A) Representative examples of colocalization events between MukB-mCherry and ParC/E-mYPet proteins within E. coli cells. The origin of replication region was identified by the binding of a TetR-CFP protein on a tetO array ( ori1 ) located 15 kb CCW of the replication origin, oriC . The fluorescence profiles of the individual cells show the distribution of fluorescence intensities. Exponential-phase cells were grown in M9-glycerol medium at 30°C prior to imaging. Cells without replication are dnaC (Ts) strains grown at the restrictive temperature for 120 min. Bars, 2 µm. (B) In order to localize the fluorescent foci corresponding to MukB and ParC/E proteins or the ori1 DNA locus within cells, microscopy images were first analyzed using the <t>MicrobeTracker</t> Suite ( http://microbetracker.org/ ) to detect and outline bacterial cells. Cumulative distributions present the distances between the centroids of MukB foci and the brightest ParC/E pixels (Materials and Methods). Colocalization (gray shaded rectangle) is defined as when the MukBEF focus centroid is 4 or less pixels (516 nm) from the brightest ParC/E pixel. (C) The percentages of colocalization between MukBEF and ParC/E were plotted in a histogram. Error bars represent the 95% confidence interval. Asterisks indicate that the measured values (meas.) are statistically significantly different from the random calculated values (rand.) (Materials and Methods).
Microbetracker Software, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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software, algorithm astra 6  (Waters Corporation)
90
Waters Corporation software, algorithm astra 6
Association of TopoIV subunits ParC and ParE with MukBEF in vivo . (A) Representative examples of colocalization events between MukB-mCherry and ParC/E-mYPet proteins within E. coli cells. The origin of replication region was identified by the binding of a TetR-CFP protein on a tetO array ( ori1 ) located 15 kb CCW of the replication origin, oriC . The fluorescence profiles of the individual cells show the distribution of fluorescence intensities. Exponential-phase cells were grown in M9-glycerol medium at 30°C prior to imaging. Cells without replication are dnaC (Ts) strains grown at the restrictive temperature for 120 min. Bars, 2 µm. (B) In order to localize the fluorescent foci corresponding to MukB and ParC/E proteins or the ori1 DNA locus within cells, microscopy images were first analyzed using the <t>MicrobeTracker</t> Suite ( http://microbetracker.org/ ) to detect and outline bacterial cells. Cumulative distributions present the distances between the centroids of MukB foci and the brightest ParC/E pixels (Materials and Methods). Colocalization (gray shaded rectangle) is defined as when the MukBEF focus centroid is 4 or less pixels (516 nm) from the brightest ParC/E pixel. (C) The percentages of colocalization between MukBEF and ParC/E were plotted in a histogram. Error bars represent the 95% confidence interval. Asterisks indicate that the measured values (meas.) are statistically significantly different from the random calculated values (rand.) (Materials and Methods).
Software, Algorithm Astra 6, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microbetracker suite  (MathWorks Inc)
90
MathWorks Inc microbetracker suite
Association of TopoIV subunits ParC and ParE with MukBEF in vivo . (A) Representative examples of colocalization events between MukB-mCherry and ParC/E-mYPet proteins within E. coli cells. The origin of replication region was identified by the binding of a TetR-CFP protein on a tetO array ( ori1 ) located 15 kb CCW of the replication origin, oriC . The fluorescence profiles of the individual cells show the distribution of fluorescence intensities. Exponential-phase cells were grown in M9-glycerol medium at 30°C prior to imaging. Cells without replication are dnaC (Ts) strains grown at the restrictive temperature for 120 min. Bars, 2 µm. (B) In order to localize the fluorescent foci corresponding to MukB and ParC/E proteins or the ori1 DNA locus within cells, microscopy images were first analyzed using the <t>MicrobeTracker</t> Suite ( http://microbetracker.org/ ) to detect and outline bacterial cells. Cumulative distributions present the distances between the centroids of MukB foci and the brightest ParC/E pixels (Materials and Methods). Colocalization (gray shaded rectangle) is defined as when the MukBEF focus centroid is 4 or less pixels (516 nm) from the brightest ParC/E pixel. (C) The percentages of colocalization between MukBEF and ParC/E were plotted in a histogram. Error bars represent the 95% confidence interval. Asterisks indicate that the measured values (meas.) are statistically significantly different from the random calculated values (rand.) (Materials and Methods).
Microbetracker Suite, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microbetracker suite/product/MathWorks Inc
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microbetracker suite - by Bioz Stars, 2026-03
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matlab r2011a software  (MathWorks Inc)
90
MathWorks Inc matlab r2011a software
Association of TopoIV subunits ParC and ParE with MukBEF in vivo . (A) Representative examples of colocalization events between MukB-mCherry and ParC/E-mYPet proteins within E. coli cells. The origin of replication region was identified by the binding of a TetR-CFP protein on a tetO array ( ori1 ) located 15 kb CCW of the replication origin, oriC . The fluorescence profiles of the individual cells show the distribution of fluorescence intensities. Exponential-phase cells were grown in M9-glycerol medium at 30°C prior to imaging. Cells without replication are dnaC (Ts) strains grown at the restrictive temperature for 120 min. Bars, 2 µm. (B) In order to localize the fluorescent foci corresponding to MukB and ParC/E proteins or the ori1 DNA locus within cells, microscopy images were first analyzed using the <t>MicrobeTracker</t> Suite ( http://microbetracker.org/ ) to detect and outline bacterial cells. Cumulative distributions present the distances between the centroids of MukB foci and the brightest ParC/E pixels (Materials and Methods). Colocalization (gray shaded rectangle) is defined as when the MukBEF focus centroid is 4 or less pixels (516 nm) from the brightest ParC/E pixel. (C) The percentages of colocalization between MukBEF and ParC/E were plotted in a histogram. Error bars represent the 95% confidence interval. Asterisks indicate that the measured values (meas.) are statistically significantly different from the random calculated values (rand.) (Materials and Methods).
Matlab R2011a Software, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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custom-written matlab software  (MathWorks Inc)
90
MathWorks Inc custom-written matlab software
Association of TopoIV subunits ParC and ParE with MukBEF in vivo . (A) Representative examples of colocalization events between MukB-mCherry and ParC/E-mYPet proteins within E. coli cells. The origin of replication region was identified by the binding of a TetR-CFP protein on a tetO array ( ori1 ) located 15 kb CCW of the replication origin, oriC . The fluorescence profiles of the individual cells show the distribution of fluorescence intensities. Exponential-phase cells were grown in M9-glycerol medium at 30°C prior to imaging. Cells without replication are dnaC (Ts) strains grown at the restrictive temperature for 120 min. Bars, 2 µm. (B) In order to localize the fluorescent foci corresponding to MukB and ParC/E proteins or the ori1 DNA locus within cells, microscopy images were first analyzed using the <t>MicrobeTracker</t> Suite ( http://microbetracker.org/ ) to detect and outline bacterial cells. Cumulative distributions present the distances between the centroids of MukB foci and the brightest ParC/E pixels (Materials and Methods). Colocalization (gray shaded rectangle) is defined as when the MukBEF focus centroid is 4 or less pixels (516 nm) from the brightest ParC/E pixel. (C) The percentages of colocalization between MukBEF and ParC/E were plotted in a histogram. Error bars represent the 95% confidence interval. Asterisks indicate that the measured values (meas.) are statistically significantly different from the random calculated values (rand.) (Materials and Methods).
Custom Written Matlab Software, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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custom software matlab  (MathWorks Inc)
90
MathWorks Inc custom software matlab
Association of TopoIV subunits ParC and ParE with MukBEF in vivo . (A) Representative examples of colocalization events between MukB-mCherry and ParC/E-mYPet proteins within E. coli cells. The origin of replication region was identified by the binding of a TetR-CFP protein on a tetO array ( ori1 ) located 15 kb CCW of the replication origin, oriC . The fluorescence profiles of the individual cells show the distribution of fluorescence intensities. Exponential-phase cells were grown in M9-glycerol medium at 30°C prior to imaging. Cells without replication are dnaC (Ts) strains grown at the restrictive temperature for 120 min. Bars, 2 µm. (B) In order to localize the fluorescent foci corresponding to MukB and ParC/E proteins or the ori1 DNA locus within cells, microscopy images were first analyzed using the <t>MicrobeTracker</t> Suite ( http://microbetracker.org/ ) to detect and outline bacterial cells. Cumulative distributions present the distances between the centroids of MukB foci and the brightest ParC/E pixels (Materials and Methods). Colocalization (gray shaded rectangle) is defined as when the MukBEF focus centroid is 4 or less pixels (516 nm) from the brightest ParC/E pixel. (C) The percentages of colocalization between MukBEF and ParC/E were plotted in a histogram. Error bars represent the 95% confidence interval. Asterisks indicate that the measured values (meas.) are statistically significantly different from the random calculated values (rand.) (Materials and Methods).
Custom Software Matlab, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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smtracker  (MathWorks Inc)
90
MathWorks Inc smtracker
Single molecule tracking analysis for RarA-YFP in recombinational and Y-polymerase mutant backgrounds. ( A ) RarA-mVenus dynamic population difference in the backgrounds studied compared to wild type in no-drug condition. ( B ) RarA-mVenus DPDs after addition of 0.5 mM H 2 O 2 , or C ) of 50 ng/ml MMC, for 60 min, compared to drug-free conditions in each background. * represent statistical significance in Z-test (included in <t>SMTracker,</t> see methods).
Smtracker, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Association of TopoIV subunits ParC and ParE with MukBEF in vivo . (A) Representative examples of colocalization events between MukB-mCherry and ParC/E-mYPet proteins within E. coli cells. The origin of replication region was identified by the binding of a TetR-CFP protein on a tetO array ( ori1 ) located 15 kb CCW of the replication origin, oriC . The fluorescence profiles of the individual cells show the distribution of fluorescence intensities. Exponential-phase cells were grown in M9-glycerol medium at 30°C prior to imaging. Cells without replication are dnaC (Ts) strains grown at the restrictive temperature for 120 min. Bars, 2 µm. (B) In order to localize the fluorescent foci corresponding to MukB and ParC/E proteins or the ori1 DNA locus within cells, microscopy images were first analyzed using the MicrobeTracker Suite ( http://microbetracker.org/ ) to detect and outline bacterial cells. Cumulative distributions present the distances between the centroids of MukB foci and the brightest ParC/E pixels (Materials and Methods). Colocalization (gray shaded rectangle) is defined as when the MukBEF focus centroid is 4 or less pixels (516 nm) from the brightest ParC/E pixel. (C) The percentages of colocalization between MukBEF and ParC/E were plotted in a histogram. Error bars represent the 95% confidence interval. Asterisks indicate that the measured values (meas.) are statistically significantly different from the random calculated values (rand.) (Materials and Methods).

Journal: mBio

Article Title: The SMC Complex MukBEF Recruits Topoisomerase IV to the Origin of Replication Region in Live Escherichia coli

doi: 10.1128/mBio.01001-13

Figure Lengend Snippet: Association of TopoIV subunits ParC and ParE with MukBEF in vivo . (A) Representative examples of colocalization events between MukB-mCherry and ParC/E-mYPet proteins within E. coli cells. The origin of replication region was identified by the binding of a TetR-CFP protein on a tetO array ( ori1 ) located 15 kb CCW of the replication origin, oriC . The fluorescence profiles of the individual cells show the distribution of fluorescence intensities. Exponential-phase cells were grown in M9-glycerol medium at 30°C prior to imaging. Cells without replication are dnaC (Ts) strains grown at the restrictive temperature for 120 min. Bars, 2 µm. (B) In order to localize the fluorescent foci corresponding to MukB and ParC/E proteins or the ori1 DNA locus within cells, microscopy images were first analyzed using the MicrobeTracker Suite ( http://microbetracker.org/ ) to detect and outline bacterial cells. Cumulative distributions present the distances between the centroids of MukB foci and the brightest ParC/E pixels (Materials and Methods). Colocalization (gray shaded rectangle) is defined as when the MukBEF focus centroid is 4 or less pixels (516 nm) from the brightest ParC/E pixel. (C) The percentages of colocalization between MukBEF and ParC/E were plotted in a histogram. Error bars represent the 95% confidence interval. Asterisks indicate that the measured values (meas.) are statistically significantly different from the random calculated values (rand.) (Materials and Methods).

Article Snippet: Cell outlines were first delineated from a phase image using the MicrobeTracker software run in Matlab (see in the supplemental material).

Techniques: In Vivo, Binding Assay, Fluorescence, Imaging, Microscopy

Single molecule tracking analysis for RarA-YFP in recombinational and Y-polymerase mutant backgrounds. ( A ) RarA-mVenus dynamic population difference in the backgrounds studied compared to wild type in no-drug condition. ( B ) RarA-mVenus DPDs after addition of 0.5 mM H 2 O 2 , or C ) of 50 ng/ml MMC, for 60 min, compared to drug-free conditions in each background. * represent statistical significance in Z-test (included in SMTracker, see methods).

Journal: Scientific Reports

Article Title: Single molecule tracking reveals functions for RarA at replication forks but also independently from replication during DNA repair in Bacillus subtilis

doi: 10.1038/s41598-018-38289-6

Figure Lengend Snippet: Single molecule tracking analysis for RarA-YFP in recombinational and Y-polymerase mutant backgrounds. ( A ) RarA-mVenus dynamic population difference in the backgrounds studied compared to wild type in no-drug condition. ( B ) RarA-mVenus DPDs after addition of 0.5 mM H 2 O 2 , or C ) of 50 ng/ml MMC, for 60 min, compared to drug-free conditions in each background. * represent statistical significance in Z-test (included in SMTracker, see methods).

Article Snippet: All U-track, MicrobeTracker and SMTracker are software running in MATLAB (Mathworks).

Techniques: Mutagenesis